7SIQ

Crystal structure of a peptide chain release factor 3 (prfC) from Stenotrophomonas maltophilia bound to GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	287
Detector technology	CCD
Collection date	2020-06-25
Detector	RAYONIX MX-300
Wavelength(s)	0.9787
Spacegroup name	P 32 2 1
Unit cell lengths	96.540, 96.540, 170.070
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.270 - 2.950
R-factor	0.2263
Rwork	0.224
R-free	0.27860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tr5
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (dev-4274)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.270	48.270	3.030
High resolution limit [Å]	2.950	13.190	2.950
Rmerge	0.068	0.027	0.732
Rmeas	0.071	0.029	0.767
Total number of observations	216402
Number of reflections	19936	266	1454
<I/σ(I)>	22.82	54.19	4
Completeness [%]	99.8	95	99.7
Redundancy	10.855	8.132	11.162
CC(1/2)	0.999	0.999	0.991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	287	StmaA.18013.a.B1.PW38802 at 20.5 mg/mL with 3 mM MgCl2 and 3 mM GDP against Morpheus screen condition D5 containing 10% PEG 20,000, 20% PEG 550 MME, 0.02 mM each 1,6-hexanediol, 1-butanol, 1,2-propanediol, 2-propanol, 1,4-butanediol, 1,3-propanediol, 0.1 M MOPES/Hepes pH 7.5, direct cryo, unique puck ID tja6-9, crystal tracking ID 315959d5