7SIJ

Myocilin OLF mutant E352K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-03-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	49.336, 50.734, 50.490
Unit cell angles	90.00, 96.60, 90.00

Refinement procedure

Resolution	37.260 - 1.540
R-factor	0.1563
Rwork	0.155
R-free	0.17780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pke
RMSD bond length	0.005
RMSD bond angle	0.867
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.260	1.595
High resolution limit [Å]	1.540	1.540
Rmerge	0.051	0.153
Rmeas	0.055	0.167
Rpim	0.022	0.066
Number of reflections	36260	3465
<I/σ(I)>	22.48	8.21
Completeness [%]	98.6	95.58
Redundancy	6.4	6.3
CC(1/2)	0.999	0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	289	10% PEG 8000, 0.25M magnesium chloride