7SGC

Replication Initiator Protein REPE54 and cognate DNA sequence with terminal five prime phosphates.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2021-04-16
Detector	RDI CMOS_8M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	109.161, 83.051, 74.349
Unit cell angles	90.00, 123.95, 90.00

Refinement procedure

Resolution	36.770 - 2.700
R-factor	0.1889
Rwork	0.186
R-free	0.24510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rva
RMSD bond length	0.011
RMSD bond angle	1.297
Data reduction software	XDS (Jan 31, 2020)
Data scaling software	XSCALE (Jan 31, 2020)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.770	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.063	0.695
Rmeas	0.075	0.822
Rpim	0.039	0.434
Number of reflections	14437	1896
<I/σ(I)>	13	1.8
Completeness [%]	95.0	93.7
Redundancy	3.6	3.5
CC(1/2)	0.997	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	30 mM MgCl2, 18% PEG 400, 220 mM Tris HCl