7SFM
Mycobacterium tuberculosis Hip1 crystal structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-09 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9436 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 104.891, 104.891, 127.802 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.464 - 2.149 |
R-factor | 0.1759 |
Rwork | 0.174 |
R-free | 0.20490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ugq |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.464 | 2.230 |
High resolution limit [Å] | 2.149 | 2.149 |
Rmeas | 0.000 | 0.000 |
Number of reflections | 44555 | 4379 |
<I/σ(I)> | 17 | |
Completeness [%] | 99.0 | |
Redundancy | 1.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 30% PEG 3350, 0.1M sodium acetate pH 5.6, 0.2 M ammonium sulfate and 10% glycerol |