7SF5

M. tb EgtD in complex with HD3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2020-08-31
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	69.146, 70.277, 143.883
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.270 - 2.520
R-factor	0.2033
Rwork	0.198
R-free	0.26760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uy5
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.270	2.610
High resolution limit [Å]	2.520	2.520
Number of reflections	24381	2384
<I/σ(I)>	11
Completeness [%]	99.9
Redundancy	1
CC(1/2)	0.980	0.550

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	0.2 M potassium phosphate dibasic and 20 % w/v polyethylene glycol 3,350