7SEY

M. tb EgtD in complex with SGH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2020-07-31
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	69.281, 69.281, 142.127
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.310 - 2.390
R-factor	0.2259
Rwork	0.220
R-free	0.28210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uy5
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.310	2.476
High resolution limit [Å]	2.390	2.390
Rmerge		1.925
Number of reflections	12689	759
<I/σ(I)>	10
Completeness [%]	88.1
Redundancy	16.9
CC(1/2)	0.990	0.634

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	0.2 M succinic acid pH 7.0 and 20 % w/v polyethylene glycol 1,000