7SER

PRMT5/MEP50 with compound 30 bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	I 2 2 2
Unit cell lengths	103.891, 138.305, 179.191
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.930 - 2.140
R-factor	0.2079
Rwork	0.206
R-free	0.25260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7s0u
RMSD bond length	0.007
RMSD bond angle	0.828
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.140	2.140
Number of reflections	61203	1684
<I/σ(I)>	16.1
Completeness [%]	85.9
Redundancy	2.8
CC(1/2)	0.990	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	100 mM Sodium Citrate pH 5.4, 12% PEG 3350, 4% Tascimate pH 5