7SED

Crystal structure of human Fibrillarin in complex with compound 2a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-29
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9537
Spacegroup name	C 2 2 21
Unit cell lengths	69.291, 139.581, 66.249
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.630 - 1.900
R-factor	0.1914
Rwork	0.189
R-free	0.22490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ipx
RMSD bond length	0.011
RMSD bond angle	1.105
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.050	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.048	0.453
Rmeas	0.050	0.474
Rpim	0.014	0.136
Number of reflections	25684	1570
<I/σ(I)>	22.6	4
Completeness [%]	99.5
Redundancy	12.8
CC(1/2)	1.000	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2.75-3.3 M Sodium Formate