7SE7

Crystal structure of human Fibrillarin in complex with S-adenosyl-L-methionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-06
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	C 2 2 21
Unit cell lengths	69.276, 138.924, 65.665
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.080 - 1.750
R-factor	0.1808
Rwork	0.179
R-free	0.20940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ipx
RMSD bond length	0.010
RMSD bond angle	1.047
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.720	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.073	0.720
Rmeas	0.078	0.777
Rpim	0.029	0.288
Number of reflections	32353	1745
<I/σ(I)>	13.2	1.9
Completeness [%]	99.9
Redundancy	7.3
CC(1/2)	0.999	0.807

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2.75-3.3 M Sodium Formate