7SE6

Crystal structure of human Fibrillarin in ligand-free state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-10
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	C 2 2 21
Unit cell lengths	69.145, 138.186, 66.551
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.330 - 1.990
R-factor	0.2283
Rwork	0.226
R-free	0.25950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ipx
RMSD bond length	0.013
RMSD bond angle	1.141
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.930	2.040
High resolution limit [Å]	1.990	1.990
Rmerge	0.053	1.087
Rmeas	0.057	1.172
Rpim	0.021	0.433
Number of reflections	22206	1623
<I/σ(I)>	23.7	1.7
Completeness [%]	99.9
Redundancy	7.3
CC(1/2)	1.000	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2.75-3.3 M Sodium Formate