7SD2
Murine Fab that recognizes Hev b 8 (profilin for Hevea brasiliensis)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-21 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 91.000, 103.845, 180.204 |
Unit cell angles | 90.00, 94.26, 90.00 |
Refinement procedure
Resolution | 44.960 - 3.750 |
R-factor | 0.2484 |
Rwork | 0.246 |
R-free | 0.28410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1baf |
RMSD bond length | 0.002 |
RMSD bond angle | 0.557 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.920 | 4.190 |
High resolution limit [Å] | 3.750 | 3.750 |
Rmerge | 0.129 | |
Rpim | 0.084 | |
Number of reflections | 17207 | 4862 |
<I/σ(I)> | 8.1 | |
Completeness [%] | 99.1 | 99.8 |
Redundancy | 3.3 | |
CC(1/2) | 0.991 | 0.843 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 291 | 0.2 M magnesium acetate tetrahydrate, 20% w/v PEG3350 |