7SCT

Crystal Structure of the Tick Evasin EVA-AAM1001 Complexed to Human Chemokine CCL16

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953
Spacegroup name	I 4 2 2
Unit cell lengths	96.258, 96.258, 84.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.380 - 1.840
R-factor	0.2035
Rwork	0.203
R-free	0.21010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7scs
RMSD bond length	0.007
RMSD bond angle	1.083
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.130	1.880
High resolution limit [Å]	1.840	1.840
Number of reflections	17690	1051
<I/σ(I)>	23
Completeness [%]	99.9
Redundancy	26
CC(1/2)	0.999	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.26 M (NH4)2SO4 (Precipitant),0.1 M HEPES 7.5 pH (Buffer)