7SCS

Crystal Structure of the Tick Evasin EVA-AAM1001 Complexed to Human Chemokine CCL11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953
Spacegroup name	P 43 21 2
Unit cell lengths	75.028, 75.028, 66.936
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.580 - 1.510
R-factor	0.1856
Rwork	0.184
R-free	0.20650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7s58
RMSD bond length	0.009
RMSD bond angle	1.210
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.580	1.540
High resolution limit [Å]	1.510	1.510
Number of reflections	56735	1433
<I/σ(I)>	23.9
Completeness [%]	99.8
Redundancy	25.6
CC(1/2)	1.000	0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M BIS-TRIS 6.5 pH (Buffer) , 2 M (NH4)2SO4 (Precipitant)