7SBU
Crystal structure of SARS-CoV-2 spike protein receptor-binding domain in complex with a highly potent antibody J08 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-1 |
Synchrotron site | SSRL |
Beamline | BL12-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.539, 103.177, 122.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.800 - 2.530 |
R-factor | 0.2224 |
Rwork | 0.220 |
R-free | 0.26110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7jmw |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.580 |
High resolution limit [Å] | 2.530 | 6.890 | 2.540 |
Rmerge | 0.128 | 0.073 | 0.501 |
Rmeas | 0.137 | 0.079 | 0.536 |
Rpim | 0.049 | 0.029 | 0.186 |
Number of reflections | 23706 | 1309 | 1144 |
<I/σ(I)> | 7.8 | ||
Completeness [%] | 99.7 | 99.5 | 97.4 |
Redundancy | 7.9 | 7 | 7.7 |
CC(1/2) | 0.995 | 0.899 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 17% (w/v) PEG 4000, 15% (v/v) Glycerol, 8.5% (v/v) Isopropanol, 0.085 M Sodium HEPES pH 7.5 |