7SBM

Human glutaminase C (Y466W) with L-Gln, open conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	98.842, 139.100, 177.435
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.290 - 2.800
R-factor	0.2018
Rwork	0.200
R-free	0.24280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d3o
RMSD bond length	0.009
RMSD bond angle	1.301
Data reduction software	HKL-2000 (721.2)
Data scaling software	HKL-2000 (721.2)
Phasing software	PHENIX (1.15.2_3472)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.300	2.850
High resolution limit [Å]	2.800	2.810
Rpim	0.061	0.251
Number of reflections	60927	5953
<I/σ(I)>	4.2
Completeness [%]	99.4	98.52
Redundancy	6.3	6.3
CC(1/2)	0.993	0.948

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291.15	11.5% PEG 6000, 0.95 M LiCl, and 0.1 M Tris pH 8.5