7SBI

Tandem diubiquitin-like domain from chicken 2'-5'-oligoadenylate synthetase-like protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	28.921, 100.922, 55.748
Unit cell angles	90.00, 104.96, 90.00

Refinement procedure

Resolution	27.940 - 2.230
R-factor	0.2307
Rwork	0.229
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Homology Model
RMSD bond length	0.002
RMSD bond angle	0.549
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (phenix-1.18.2-3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.530	2.290
High resolution limit [Å]	2.230	2.230
Rpim	0.075	1.100
Number of reflections	13956	1176
<I/σ(I)>	8.3	1.3
Completeness [%]	93.8	79.6
Redundancy	2.9	2.3
CC(1/2)		0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	tri-ammonium citrate, PEG 3350, galactose