7SB8

d(GA(CGA)5) parallel-stranded homo-duplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-12
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	19.677, 30.422, 180.822
Unit cell angles	90.00, 90.41, 90.00

Refinement procedure

Resolution	90.409 - 1.317
R-factor	0.1734
Rwork	0.171
R-free	0.21390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ixj
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.410	1.340
High resolution limit [Å]	1.317	1.317
Rmerge	0.093	0.669
Rmeas	0.106	0.757
Rpim	0.049	0.350
Number of reflections	48232	2175
<I/σ(I)>	9.9	2
Completeness [%]	94.7
Redundancy	4.5
CC(1/2)	0.995	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	295	8% PEG400, 96 mM strontium chloride, 32 mM lithium chloride, 8 mM hexamminecobalt(III) chloride, 24 mM sodium cacodylate, equilibrated against 30% PEG400