7SB6
Crystal Structure of Ancestral Mammalian Cadherin-23 EC1-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 118.446, 168.834, 125.276 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.529 - 2.588 |
| Rwork | 0.204 |
| R-free | 0.24610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2whv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.554 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.630 |
| High resolution limit [Å] | 2.588 | 2.588 |
| Rmerge | 0.091 | 1.022 |
| Number of reflections | 39416 | 1925 |
| <I/σ(I)> | 18.1 | 1.75 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 6.1 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 3.5 M Ammonium chloride 0.1 M MES pH 6.5 |
