7SAL

Crystal Structure of LaM6 Nanobody bound to mCherry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-02
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 1 21 1
Unit cell lengths	74.083, 87.948, 74.634
Unit cell angles	90.00, 109.49, 90.00

Refinement procedure

Resolution	49.970 - 1.930
R-factor	0.1621
Rwork	0.161
R-free	0.19100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h5q
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.990
High resolution limit [Å]	1.920	4.140	1.920
Rmerge	0.090	0.055	0.949
Rmeas	0.097	0.059	1.046
Rpim	0.034	0.020	0.425
Total number of observations	492694
Number of reflections	66672	6596	6712
<I/σ(I)>	10.1
Completeness [%]	98.8	95.8	99.1
Redundancy	7.4	7.6	5.7
CC(1/2)		0.997	0.528

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	295	100mM MIB buffer with 25% (w/v) PEG1500