7SAJ

Crystal Structure of LaM2 Nanobody bound to mCherry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	171.437, 160.560, 88.786
Unit cell angles	90.00, 109.02, 90.00

Refinement procedure

Resolution	71.000 - 2.370
R-factor	0.2081
Rwork	0.208
R-free	0.23410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h5q
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	71.000	50.000	2.330
High resolution limit [Å]	2.250	4.850	2.250
Rmerge	0.063	0.025	0.864
Rmeas	0.074	0.030	1.019
Rpim	0.039	0.015	0.534
Number of reflections	106342	10781	10673
<I/σ(I)>	8.9
Completeness [%]	99.1	99.3	99.5
Redundancy	3.6	3.6	3.6
CC(1/2)		0.999	0.525

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	2.5M malonate