7SAI

Crystal Structure of Lag30 Nanobody bound to eGFP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	I 4 2 2
Unit cell lengths	173.172, 173.172, 92.288
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.940 - 2.230
R-factor	0.1724
Rwork	0.171
R-free	0.19710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4eul
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.280
High resolution limit [Å]	2.200	4.740	2.200
Rmerge	0.146	0.088	1.791
Rmeas	0.151	0.092	1.859
Rpim	0.041	0.025	0.496
Number of reflections	65199	3624	3353
<I/σ(I)>	7.5
Completeness [%]	100.0	100	100
Redundancy	13.6	13	13.9
CC(1/2)		0.997	0.504

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	800 mM KH2PO4/NaH2PO4, 100 mM HEPES