7SAF

Fragment of streptococcal M87 protein fused to GCN4 adaptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	97.223, 97.223, 40.681
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.480 - 2.450
R-factor	0.2581
Rwork	0.257
R-free	0.27170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kql
RMSD bond length	0.007
RMSD bond angle	0.870
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.480	2.538
High resolution limit [Å]	2.450	2.450
Rmerge	0.387	4.684
Rmeas	0.404	4.911
Rpim	0.117	1.459
Number of reflections	7580	738
<I/σ(I)>	31.4	0.78
Completeness [%]	99.8
Redundancy	12.1
CC(1/2)	0.987	0.556

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.6 M monobasic ammonium phosphate, 0.1 M Tris-HCl, pH 8.0