7S96

Structure of the Light Harvesting Complex PC577 from Hemiselmis pacifica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-24
Detector	DECTRIS PILATUS3 R 300K
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	41.381, 95.886, 125.003
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.710 - 1.800
R-factor	0.1774
Rwork	0.175
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lm6
RMSD bond length	0.005
RMSD bond angle	0.764
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.080	0.040	0.664
Rmeas	0.092	0.043	0.809
Rpim	0.044	0.016	0.455
Total number of observations	184915
Number of reflections	46623	2579	2170
<I/σ(I)>	7.7
Completeness [%]	98.9	99.9	94.3
Redundancy	4	7	2.8
CC(1/2)		0.999	0.686

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25% 3350 PEG, 50 mM HEPES, 100 mM MgCl2