7S94

Structure of the core postfusion porcine endogenous retrovirus fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9201
Spacegroup name	P 1 21 1
Unit cell lengths	96.860, 34.219, 100.445
Unit cell angles	90.00, 99.95, 90.00

Refinement procedure

Resolution	47.700 - 2.000
R-factor	0.1767
Rwork	0.174
R-free	0.21680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jgs
Data reduction software	DIALS
Data scaling software	Aimless (0.7.3)
Phasing software	MoRDa
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.730	47.730	2.050
High resolution limit [Å]	2.000	8.930	2.000
Rmerge	0.162	0.110	0.605
Rmeas	0.175	0.117	0.688
Rpim	0.066	0.041	0.316
Total number of observations		4114	8291
Number of reflections	41923	566	1970
<I/σ(I)>	7.3	11.2	3.1
Completeness [%]	93.4	98.9	60.4
Redundancy	6.6	7.3	4.2
CC(1/2)	0.984	0.991	0.653

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	24% (w/v) PEG 1500 and 20% (v/v) glycerol