7S8Z

Room-temperature apo Human Hsp90a-NTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	278
Detector technology	PIXEL
Collection date	2020-09-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	66.099, 89.880, 101.306
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.890 - 1.640
R-factor	0.151
Rwork	0.150
R-free	0.16830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yer
RMSD bond length	0.011
RMSD bond angle	1.220
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19rc5_4047)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.890	1.700
High resolution limit [Å]	1.640	1.640
Rmerge	0.092	0.092
Number of reflections	35591	3593
<I/σ(I)>	9.91
Completeness [%]	95.4
Redundancy	4.7
CC(1/2)	0.987	0.469

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	100 mM HEPES, pH 7 and 1.6 sodium citrate dihydrate