7S8G

Structure of anti-LASV Fab 25.10C with FNQI mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03317
Spacegroup name	P 1 21 1
Unit cell lengths	71.600, 72.718, 90.014
Unit cell angles	90.00, 113.27, 90.00

Refinement procedure

Resolution	65.780 - 2.570
R-factor	0.2246
Rwork	0.220
R-free	0.26270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ulu
RMSD bond length	0.003
RMSD bond angle	0.606
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.780	2.610
High resolution limit [Å]	2.566	2.566
Number of reflections	27076	1338
<I/σ(I)>	5.4
Completeness [%]	98.8
Redundancy	3.3
CC(1/2)	0.995	0.564

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293.15	0.1 M MES/sodium hydroxide pH 6.0, 40% (v/v) PEG 400, and 5% (w/v) PEG 3000