7S7W
Crystal structure of iNicSnFR3a Nicotine Sensor precursor binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.7749 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.801, 61.215, 58.803 |
| Unit cell angles | 90.00, 100.44, 90.00 |
Refinement procedure
| Resolution | 31.540 - 1.100 |
| R-factor | 0.1732 |
| Rwork | 0.173 |
| R-free | 0.19820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7s7t |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.770 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.130 | 1.120 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rpim | 0.020 | 0.876 |
| Number of reflections | 114191 | 5453 |
| <I/σ(I)> | 14.1 | 0.9 |
| Completeness [%] | 96.8 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 296 | 0.2 M Ammonium acetate 0.1 M BisTris, pH 6.5 25% PEG 3,350 |
