7S7V
Crystal structure of iNicSnFR3a Fluorescent Nicotine Sensor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9848 |
| Spacegroup name | P 1 |
| Unit cell lengths | 81.189, 86.865, 91.830 |
| Unit cell angles | 75.72, 85.56, 85.28 |
Refinement procedure
| Resolution | 40.390 - 2.500 |
| R-factor | 0.2327 |
| Rwork | 0.232 |
| R-free | 0.27460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7s7t |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.390 | 2.500 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rpim | 0.060 | 0.466 |
| Number of reflections | 82648 | 4588 |
| <I/σ(I)> | 8.3 | 1.7 |
| Completeness [%] | 98.7 | 98.1 |
| Redundancy | 3.5 | 3.2 |
| CC(1/2) | 0.978 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 296 | 12.5% PEG 1,000 12.5% PEG 3,350 12.5% MPD 0.02 M of each amino acid 0.1 M MOPS/HEPES-Na, pH 7.5 |
