7S69

N-acetylglucosamine-1-phosphotransferase (GNPT) gamma subunit (GNPTG), from clawed frog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	50.317, 61.684, 163.029
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.190 - 3.040
R-factor	0.1956
Rwork	0.191
R-free	0.24170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2lvx
RMSD bond length	0.004
RMSD bond angle	0.669
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.040	3.040
Rmeas	0.136
Number of reflections	8751	577
<I/σ(I)>	12.7	1.29
Completeness [%]	85.0
Redundancy	8
CC(1/2)		0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	20% PEG 3350, 0.1M bis-tris propane pH8, 0.2M KNO3