7S5B
Unbound State of a De novo designed Protein Binder to the Human Interleukin-7 Receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033310 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 92.226, 92.226, 108.436 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.150 - 1.500 |
| R-factor | 0.178 |
| Rwork | 0.177 |
| R-free | 0.19830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | model |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.389 |
| Data reduction software | XDS (20200131) |
| Data scaling software | XDS (20200131) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.18rc5_3822) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.150 | 1.554 |
| High resolution limit [Å] | 1.500 | 1.501 |
| Rmerge | 0.046 | 1.610 |
| Rmeas | 0.049 | |
| Rpim | 0.019 | 0.671 |
| Number of reflections | 28231 | 2735 |
| <I/σ(I)> | 16.19 | 1.37 |
| Completeness [%] | 99.0 | 97.35 |
| Redundancy | 6.7 | 6.5 |
| CC(1/2) | 0.999 | 0.450 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 3.0 M sodium malonate pH 7.0 |
