7S4G

Fab fragment bound to the Cter peptide of Ly6G6D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-05-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	69.286, 133.389, 100.038
Unit cell angles	90.00, 90.03, 90.00

Refinement procedure

Resolution	35.050 - 2.200
R-factor	0.194
Rwork	0.192
R-free	0.23480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fve
RMSD bond length	0.012
RMSD bond angle	1.171
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.820	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.159	1.109
Number of reflections	90064	8758
<I/σ(I)>	10.2
Completeness [%]	97.7
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	292	0.1M HEPES pH 7, 15% PEG4000