7S3L

Structure of Bacillus subtilis CcrZ (previously called YtmP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9784
Spacegroup name	P 43 21 2
Unit cell lengths	98.118, 98.118, 69.127
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.690 - 2.600
R-factor	0.2445
Rwork	0.242
R-free	0.26290
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.039
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	98.120	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.146	1.598
Number of reflections	10890	1299
<I/σ(I)>	14.8	2.6
Completeness [%]	100.0	100
Redundancy	25.5	27.2
CC(1/2)	0.998	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.84	298	30% PEG 6000, 1 M LiCl, 0.1 M NaOAc (pH 5.84)