7S23

Crystal structure of alpha-COP-WD40 domain, Y139A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.92
Spacegroup name	P 1 21 1
Unit cell lengths	37.315, 171.553, 71.281
Unit cell angles	90.00, 99.71, 90.00

Refinement procedure

Resolution	65.020 - 1.490
R-factor	0.142
Rwork	0.140
R-free	0.18400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j87
RMSD bond length	0.005
RMSD bond angle	0.696
Data reduction software	HKL-3000
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	171.550	1.520
High resolution limit [Å]	1.490	1.490
Rmerge	0.086	0.710
Number of reflections	140251	6840
<I/σ(I)>	5	1.1
Completeness [%]	97.9
Redundancy	3.6
CC(1/2)	0.996	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295.15	18% PEG3350 and 0.2M Potassium-sodium tartarate