7S1O

Structure of human POT1C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-19
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54178
Spacegroup name	P 1 21 1
Unit cell lengths	61.980, 61.800, 83.740
Unit cell angles	90.00, 94.50, 90.00

Refinement procedure

Resolution	28.980 - 2.550
R-factor	0.223
Rwork	0.220
R-free	0.27140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5un7
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.620
High resolution limit [Å]	2.550	2.550
Number of reflections	20551	1266
<I/σ(I)>	13.92
Completeness [%]	98.3
Redundancy	7.3
CC(1/2)	0.970	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	200 mM DL-Malic acid pH 7.0, 30% v./v. PEG600 and 6% methanol