7S08

Crystal structure of Epstein-Barr virus gH/gL targeting antibody 770F7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	187.194, 40.222, 135.151
Unit cell angles	90.00, 110.54, 90.00

Refinement procedure

Resolution	44.620 - 2.420
R-factor	0.2148
Rwork	0.212
R-free	0.26040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qhk
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.500
High resolution limit [Å]	2.400	2.400
Number of reflections	22877	1928
<I/σ(I)>	8.7
Completeness [%]	95.4
Redundancy	4.5
CC(1/2)	0.850	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M HEPES PH7.5, 0.1M CaCl2, 25% PEG3350, 4% isopropanol