7S01

X-ray structure of the phage AR9 non-virion RNA polymerase holoenzyme in complex with a forked oligonucleotide containing the P077 promoter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.91840
Spacegroup name	C 1 2 1
Unit cell lengths	176.925, 110.455, 222.379
Unit cell angles	90.00, 98.52, 90.00

Refinement procedure

Resolution	49.350 - 3.400
R-factor	0.2415
Rwork	0.239
R-free	0.29260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	EMD-24763
RMSD bond length	0.002
RMSD bond angle	0.469
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	3.830	3.580
High resolution limit [Å]	3.380	3.580	3.380
Rmeas	0.157	0.614	1.013
Number of reflections	59745	8976	9572
<I/σ(I)>	7.94	2.1	1.2
Completeness [%]	94.0	97	83.9
Redundancy	2.88	2.99	2.58
CC(1/2)	0.993	0.848	0.664

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	1.5 ul aliquot of the AR9 nvRNAP promoter complex (10 mg/mL) was mixed with the same volume of a solution containing 150 mM MIB pH 5, 150 mM LiCl, 13 % PEG 1500 and incubated as a hanging drop over the same solution.