7RZZ
Crystal structure of FBF-2 in complex with LST-1 site A peptide and compact FBE RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 |
| Unit cell lengths | 93.169, 93.169, 111.278 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.700 - 2.390 |
| R-factor | 0.1751 |
| Rwork | 0.171 |
| R-free | 0.21510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k5q |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.446 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.700 | 2.480 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rpim | 0.031 | 0.186 |
| Number of reflections | 26102 | 2118 |
| <I/σ(I)> | 14.5 | |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30% (v/v) PEG 400, 0.1 M CHES, pH 9.5 |
