7RYW
Crystal structure of Ferritin grown by the microbatch method in the presence of Agarose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 19-ID |
| Synchrotron site | NSLS-II |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-02 |
| Detector | ADSC HF-4M |
| Wavelength(s) | 0.979 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.507, 181.507, 181.507 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.700 - 2.090 |
| R-factor | 0.1713 |
| Rwork | 0.170 |
| R-free | 0.19580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w0o |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.773 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.700 | 2.150 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.112 | |
| Number of reflections | 15632 | 1090 |
| <I/σ(I)> | 0.935 | |
| Completeness [%] | 99.8 | |
| Redundancy | 20 | |
| CC(1/2) | 1.000 | 0.954 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 290 | CdSO4, NH4SO4, Tris pH 7.4 |
