7RYV
Anti-HIV neutralizing antibody Ab1573 Fab isolated from sequentially immunized macaques
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-05-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.502, 138.396, 83.727 |
| Unit cell angles | 90.00, 95.71, 90.00 |
Refinement procedure
| Resolution | 38.650 - 2.500 |
| R-factor | 0.2172 |
| Rwork | 0.216 |
| R-free | 0.25370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ryu |
| Data reduction software | TRUNCATE |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.820 | 2.280 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Number of reflections | 170333 | 8678 |
| <I/σ(I)> | 5.2 | 1.6 |
| Completeness [%] | 99.0 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.970 | 0.520 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293.15 | 0.1M Tris (pH 8.2), 26% (w/v) PEG 4000 |
