7RY2
mSandy2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-05 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.11 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.680, 147.210, 240.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.500 - 2.050 |
| R-factor | 0.1913 |
| Rwork | 0.190 |
| R-free | 0.22270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h5q |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.665 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.310 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rpim | 0.052 | 0.890 |
| Number of reflections | 133939 | 6432 |
| <I/σ(I)> | 11.9 | 1 |
| Completeness [%] | 99.9 | 97.3 |
| Redundancy | 13 | 10.9 |
| CC(1/2) | 0.999 | 0.396 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 100 mM Tris buffer (pH 8.5), 100 mM NaCl, 20% PEG-3350, 34 mg/mL protein |
