7RVK

Segment from Y169G mutant of the human prion protein 169-175 GSNQNNF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ELECTRON MICROSCOPE
Source details	OTHER
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-09-22
Detector	TVIPS TEMCAM-F416
Wavelength(s)	0.0251
Spacegroup name	P 1
Unit cell lengths	4.860, 14.110, 18.410
Unit cell angles	90.00, 93.71, 101.21

Refinement procedure

Resolution	13.840 - 1.000
R-factor	0.2024
Rwork	0.198
R-free	0.23820
Structure solution method	AB INITIO PHASING
RMSD bond length	0.010
RMSD bond angle	0.940
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	13.840	13.840	1.030
High resolution limit [Å]	1.000	4.470	1.000
Rmerge	0.238	0.159	0.430
Rmeas	0.257	0.171	0.485
Total number of observations	12907
Number of reflections	2076	18	123
<I/σ(I)>	5.05	7.72	2.25
Completeness [%]	80.3	69.2	64.7
Redundancy	6.217	6.722	4.26
CC(1/2)	0.980	0.976	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	10% w/v PEG8000, 0.1 M MES, pH 6, 0.2 M sodium acetate