7RVE

Segment from the S170N mutant of the human prion protein 168-176 EYNNQNNFV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ELECTRON MICROSCOPE
Source details	OTHER
Temperature [K]	100
Detector technology	CMOS
Collection date	2017-04-25
Detector	TVIPS TEMCAM-F416
Wavelength(s)	0.0251
Spacegroup name	P 1
Unit cell lengths	4.930, 10.140, 31.560
Unit cell angles	94.13, 90.59, 102.74

Refinement procedure

Resolution	9.860 - 0.850
R-factor	0.2177
Rwork	0.213
R-free	0.26630
Structure solution method	AB INITIO PHASING
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	9.860	9.860	0.870
High resolution limit [Å]	0.850	3.800	0.850
Rmerge	0.185	0.139	0.505
Rmeas	0.211	0.156	0.631
Total number of observations	16649
Number of reflections	4694	54	161
<I/σ(I)>	3.81	8.43	1.22
Completeness [%]	89.8	87.1	43.2
Redundancy	3.547	4.241	2.435
CC(1/2)	0.985	0.972	0.617

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	0.1 M sodium acetate, pH 4.5, 1 M sodium chloride, 0.1 M lithium sulfate