7RVD

Segment from the mouse/cow prion protein 168-176 QYSNQNNFV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ELECTRON MICROSCOPE
Source details	OTHER
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-11-13
Detector	TVIPS TEMCAM-F416
Wavelength(s)	0.0251
Spacegroup name	P 1
Unit cell lengths	4.870, 10.170, 31.290
Unit cell angles	94.65, 90.73, 101.15

Refinement procedure

Resolution	10.391 - 1.003
R-factor	0.2056
Rwork	0.201
R-free	0.24990
Structure solution method	AB INITIO PHASING
RMSD bond length	0.008
RMSD bond angle	0.920
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	10.391	10.391	1.030
High resolution limit [Å]	1.000	4.470	1.000
Rmerge	0.167	0.171	0.514
Rmeas	0.197	0.194	0.650
Total number of observations	8918
Number of reflections	2977	30	215
<I/σ(I)>	3.99	7.81	1.33
Completeness [%]	93.9	88.2	94.7
Redundancy	2.996	3.333	2.433
CC(1/2)	0.988	0.975	0.742

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	0.5 M zinc acetate, 15% ethanol, 0.2 M MES, pH 6