7RTY
Crystal Structure of PsfC from Pseudomonas syringae PB-5123
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-08 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.12713 |
| Spacegroup name | I 41 |
| Unit cell lengths | 106.831, 106.831, 83.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.963 |
| R-factor | 0.21848 |
| Rwork | 0.216 |
| R-free | 0.25691 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.920 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.548 | 1.970 |
| High resolution limit [Å] | 1.960 | 1.963 |
| Rmerge | 0.090 | 0.919 |
| Rmeas | 0.096 | |
| Number of reflections | 30430 | 233 |
| <I/σ(I)> | 15.6 | 2.3 |
| Completeness [%] | 91.2 | 68.9 |
| Redundancy | 7.8 | 6 |
| CC(1/2) | 0.997 | 0.752 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.17-0.20 M sodium fluoride, 18-20% PEG 3,350, 0.1 M bis-tris propane pH 8.5 |
