7RS4
Crystal Structure of the ER-alpha Ligand-binding Domain (L372S, L536S) in complex with DMERI-8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.087, 58.816, 93.709 |
| Unit cell angles | 86.88, 75.21, 63.40 |
Refinement procedure
| Resolution | 90.390 - 1.780 |
| R-factor | 0.1989 |
| Rwork | 0.196 |
| R-free | 0.25090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 2QXS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.382 |
| Data reduction software | AutoProcess |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.390 | 2.020 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Number of reflections | 49778 | 2490 |
| <I/σ(I)> | 9 | |
| Completeness [%] | 54.3 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.997 | 0.553 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 298 | 20-25% PEG 3350, 200 mM MgCl2, 0.1 M Bis-Tris/Hepes/Tris-HCl |
