7RRM

Structure of the human TMED1 (p24gamma1) Golgi dynamics Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9786
Spacegroup name	P 6 2 2
Unit cell lengths	151.890, 151.890, 56.170
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.160 - 1.720
R-factor	0.1957
Rwork	0.194
R-free	0.22160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gu5
RMSD bond length	0.007
RMSD bond angle	1.411
Data reduction software	XDS (APP3)
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.160	1.820
High resolution limit [Å]	1.720	1.720
Number of reflections	41068	6485
<I/σ(I)>	19.76	0.98
Completeness [%]	99.9	99.5
Redundancy	39.64	39.69
CC(1/2)	1.000	0.490

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	294	0.1 M sodium acetate trihydrate pH 4.6, 8% (w/v) PEG 4,000