7RRI

Crystal structure of fast switching S142A/M159T mutant of fluorescent protein Dronpa (Dronpa2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	79.412, 85.809, 143.167
Unit cell angles	90.00, 95.01, 90.00

Refinement procedure

Resolution	37.290 - 2.643
R-factor	0.2292
Rwork	0.226
R-free	0.28390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uts
RMSD bond length	0.008
RMSD bond angle	1.417
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.13rc2_2986)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.290	2.738
High resolution limit [Å]	2.643	2.643
Rmerge	0.277	1.600
Rmeas	0.289	1.670
Rpim	0.079	0.460
Number of reflections	54577	5081
<I/σ(I)>	7	1.2
Completeness [%]	94.7	90.8
Redundancy	13.2	12.8
CC(1/2)	0.995	0.747

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	292	0.1 M Tris-HCl pH 7.4, 0.1 M MgCl2, 18% PEG 3350