7RRH

Crystal structure of fast switching R66M/M159T mutant of fluorescent protein Dronpa (Dronpa2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	186.629, 71.536, 108.271
Unit cell angles	90.00, 110.12, 90.00

Refinement procedure

Resolution	37.768 - 1.747
R-factor	0.1777
Rwork	0.176
R-free	0.20220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uts
RMSD bond length	0.008
RMSD bond angle	1.328
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.13rc2_2986)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.770	1.813
High resolution limit [Å]	1.747	1.747
Rmerge	0.069	1.850
Rmeas	0.071	1.920
Rpim	0.019	0.530
Number of reflections	132790	12922
<I/σ(I)>	18.4	1.2
Completeness [%]	98.2	96.4
Redundancy	13.5	12.8
CC(1/2)	1.000	0.714

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	292	0.1 M Tris-HCl pH 7.4, 0.1 M MgCl2, 18% PEG 3350