7RQR

Structure of chimeric antibody L9heavy_F10light with PfCSP peptide NANPNVDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9786
Spacegroup name	P 21 21 21
Unit cell lengths	71.503, 85.817, 145.456
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.830 - 2.230
R-factor	0.1914
Rwork	0.189
R-free	0.23840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rqp
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.490	48.490	2.300
High resolution limit [Å]	2.230	8.920	2.230
Rmerge	0.191	0.053	1.228
Rmeas	0.208	0.059	1.332
Rpim	0.081	0.025	0.509
Total number of observations	288257	4109	25607
Number of reflections	44243	767	3898
<I/σ(I)>	9	20.1	2.2
Completeness [%]	99.6	96.9	97.1
Redundancy	6.5	5.4	6.6
CC(1/2)	0.996	0.998	0.648

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M MES, pH 6.5, 18% PEG 4K, 0.6M NaCl