7RQD

Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site deacylated tRNA analog CACCA, P-site MTI-tripeptidyl-tRNA analog ACCA-ITM, and chloramphenicol at 2.50A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-05
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97895
Spacegroup name	P 21 21 21
Unit cell lengths	209.710, 449.950, 621.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	224.980 - 2.500
R-factor	0.2315
Rwork	0.229
R-free	0.27960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4o
RMSD bond length	0.005
RMSD bond angle	0.886
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	224.980	224.980	2.560
High resolution limit [Å]	2.500	11.180	2.500
Rmerge	0.160	0.036	1.472
Rmeas	0.180	0.040	1.685
Total number of observations	9083103
Number of reflections	1966210	23146	136889
<I/σ(I)>	8.41	28.81	0.85
Completeness [%]	98.5	98.5	93.1
Redundancy	4.62	5.24	3.866
CC(1/2)	0.990	0.998	0.160

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME